Sensitivity Of Duplex Real Time Pcr - 1617 Words

Sensitivity of duplex Real-time-PCR compared microscopic detection of M. bovis: Results revealed that out of 600 lymph node sample with lesions suggestive to tuberculosis 580 (96.6%) was positive for AFB detected by microscopic examination of ZN stained smears. However, by duplex real-time PCR 588 (98%) was confirmed to M. bovisinfection. Analytical specificity: The specificity of real-time PCR targeting IS1081 and IS6110 was evaluated to 19 strains of different Mycobacterial species. The real-time PCR targeting both IS1081 and IS6110 sequences showed negative result with all Mycobacterial A. Selim et el. 50 species in two concentrations of DNA from each strain, 5ng/ µl and 5pg/ µl; while strong positive result with M. bovisBCG was detected. Furthermore, ÃŽ ²-actin internal control showed positive Ct-values with all Mycobacterial species including M. bovisBCG (table 1). Table 1. Mycobacteria and non-mycobacteria analyzed for the determination of the specificity of real-time MAP-PCR Target sequence IS1801 IS6110 Template concentration Species, Sub-species Type Host species / Source 5ng/ µl 5pg/ µl 5ng/ µl 5pg/ µl M. avium subspecies avium (M128/2) TS Cattle – – – – (01A1077/2) FI-J Cattle – – – – (00A0720/2) FI-J Pig – – – – (03A0910/2) FI-J Poultry – – – – (03A2530/1) FI-J Poultry – – – – M. avium subspecies hominisuis (01A0554/1) FI-J Pig – – – – (01A1054/1) FI-J Human – – – – (01A0255/1) FI-J Dog – – – – M. bovisBCGShow MoreRelatedEvaluation Of The Method Of Rt Pcr3051 Words   |  13 PagesIntroduction RT PCR is one of the most useful methods used within investigated tissue to compare all transcripts accurately. To apply it accurately it has some challenges as it critically depends on the various reference materials and correct use of calibration. PCR diagnostics application to tissues is often limited by the lack of quality template due to inefficient RNA extraction methodologies (Sellars et al., 2007). Ecologically influenced or disease phenotypes and the cellular expression patternsRead MoreMicrorna Essay1004 Words   |  5 Pagesnucleocytoplasmic shuttle exports pre-miRNA from nucleus to cytoplasm using GTP.[24] Cytoplasmic processing Another enzyme RNase III enzyme Dicer cleaves premiRNA. It cuts away the loop joining the 3’ and 5 ‘arms yielding imperfect miRNA duplex about 22 nucleotides. Functional miRNA is duplex but only one strand is usually incorporated into RISC. [25, 26] RISC in association with miR binds to 3’ untranslated region (UTR) of target gene mRNA to inhibit protein translation or promote RNA degradation. Role of miRNARead MoreDr. Alwine : A Scientist Of Cancer Biology And An Investigator1048 Words   |  5 Pagesis a straight forward method. Several of the times this technique is used as a confirmation or verification of their research process. The Northern Blotting technique is a multipurpose procedure by allowing the usage of various types of probes. These probes include: radiolabeled and non-radiolabeled, oligonucleotides, such as primers, and in vitro transcribed RNA. This technique can also produce sequences then can be partial homology, unlike real time PCR or other procedures which can be used as hybridizationRead MoreUses of Nanotechnology1790 Words   |  8 PagesINTRODUCTION: In recent times, infectious diseases continue to pose a major healthcare issue in developing countries making it imperative to develop logical solutions for robust and rapid diagnosis and treatment of these infections. Traditional techniques suffer from limitations, including laborious specimen processing, bulky instrumentation, and slow result readout. In view of the urgency for sensitive, specific, robust and rapid diagnostics, numerous advancements have been made in the areaRead MoreCell Free DNA Case Study1819 Words   |  8 Pagesare various methods and technologies used for quantitative and qualitative analysis of ctDNAs, commonly used platforms to name a few are digital PCR (dPCR)283, droplet digital PCR (ddPCR)284, BEAMing285, 286, cancer personalized profiling by deep sequencing (CAPP-Seq)287, tagged amplicon deep sequencing (TAM-Seq)270, safe-sequencing (Safe-Seq)288, duplex sequencing289, integrated digital error suppression (iDES)-enhanced CAPP-Seq290, whole-genome sequencing (WGS)291, 292 and next-generation sequencing

Don t Be A Sucker Essay - 1815 Words

Even after war, groups will always produce media with the intent of influencing the public. â€Å"Meet King Joe,† for example, is a post World War II film that follows Joe on an educational journey where he learns about how the American economy works to make his life better. The other post World War II film to be analyzed, â€Å"Don’t Be a Sucker,† follows Mike as a wise stranger tells him about how, as Americans, we should all be tolerant of each others differences lest we become like Nazi Germany. Both of these films utilize Plain Folks appeals and other techniques in an effort to influence public opinion; but one is black, deceptive, and unethical propaganda while the other may be closer to a rational attempt at persuasion than a piece of propaganda. These films differ in many ways, but let us start with how they are the same: the use of Plain Folks appeals. In â€Å"Meet King Joe,† the titular character is just your average, everyday shmuck that is mea nt to represent the average American male in the labor force. Joe talks with a pseudo-Brooklyn accent, he is not all that sharp, and he loves buying consumer goods with his fat, American paycheck (Meet King Joe). The other film, â€Å"Don’t Be a Sucker,† aims a little higher with its main character. Instead of your average blue-collar worker, we see what might be described as your average white-collar worker. The man is dressed well, just coming off of a train presumably to or from his office job when the audience first sees him (Don t BeShow MoreRelatedThe Handsomest Drowned Man In The World Essay1207 Words   |  5 Pageswater; others a grand metamorphosis. Humans the center of this cycle, find themselves changing every day, biologically, developmentally, ideologically, etcetera. Gabriel Garcia Marquez’s â€Å"The Handsomest Drowned Man In The World,† and Carson McCuller’s â€Å"Sucker† are both excellent examples of short stories that, d espite having undeniably different plots, feature the overarching theme of transformation: the propellant through life. Transitions come in many forms, physical evolutions being the most obviousRead MoreComparing Adolescence And Maturity During The Short Stories Sucker And Araby 2084 Words   |  9 PagesComparing Adolescence and Maturity in the Short Stories â€Å"Sucker† and â€Å"Araby† Adolescence emanates from the Latin verb adolescere which means to grow into maturity. In other words, it is the period in which an individual must establish a sense of personal identity. In the Carson McCullers’s story â€Å"Sucker† two young characters struggle with their transition to adulthood. The narrator Pete, is 16, and the older of the two boys by about three years. The stories action covers an extended flashback inRead MoreSucker By James Joyce Analysis2089 Words   |  9 Pagesof personal identity. In the Carson McCullers’s story â€Å"Sucker† two young characters struggle with their transition to adulthood. The narrator Pete, is 16, and the older of the two boys by about three years. The stories action covers an extended flashback in which Pete reflects on his relationship with his cousin Sucker, who has lived with him since his â€Å"folks were killed in a wreck when he was a baby† (McCullers 2). Pete generally treats sucker poorly cau sing the latter to isolate himself, howeverRead MoreJames Joyces Story Araby, By Carson Mccullers2068 Words   |  9 Pagesgoals and convictions. In the Carson McCullers’s story â€Å"Sucker† two young characters struggle with their transition to adulthood. The narrator Pete, is 16, and the older of the two boys by about three years. The stories action covers an extended flashback in which Pete reflects on his relationship with his cousin Sucker, who has lived with him since his â€Å"folks were killed in a wreck when he was a baby† (McCullers 2). Pete generally treats sucker poorly causing the latter to isolate himself, howeverRead MoreAhmad Man Vs. Bunny Land1487 Words   |  6 Pagesoutfit that looks like a bunch of clocks on him.) I am here to take over your planet.† â€Å" Not if I have anything to do with it †said Ahmad Man. Ahmad man throws one strong punch sucker punch that made him drift off to the ground. All a sudden Time man the time traveler gets up flies over to Ahmad Man and hits him with a sucker punch. Ahmad Man ate the punch like a beast he is, and gave Time traveler the time man a grunt. Time man got a face that looked like he was horrified. Ahmad Man gave him a strongRead MoreHigh School Is The Best Four Years Of Your Life859 Words   |  4 Pagesm a sucker for a funny guy. I didn t mind us just being friends, I valued our friendship more. Until one day, he randomly asked me to be his girlfriend. I had no idea that he felt the same way that I did, so of course I said yes with no hesitation. Months went by and we fell in love with each other. I had dated other guys before but he gave me a feeling that I never felt before. I had never felt better and I knew I didn t want to be with anyone else, even though we were young. It wasn t justRead MoreCommon Mistakes in Writing1362 Words   |  6 PagesCommon Mistakes in Writing: Comma Splice In your writing, there are 3 major problems that we commonly have to address as teachers/instructors/professors/etc. I want you to take the time to read about these. While we don t expect this to fix your issues instantly, we are hoping that you ll aim to make less of these mistakes in the future (especially on your papers, where these errors WILL be counted off for).   Comma Splice -- Commas are tricky because there are so many different ways you canRead MoreWhat Do I Always Say About Hallmark?1587 Words   |  7 PagesHamlin? And it also meant a lot to me to work with Angela Lansbury on Murder She Wrote. Ms. Lansbury is a class act. She wasn t that young when she was doing that show so they tried to keep her days short, but she made a point of staying on set to do both sides of the close-up --meaning she stayed to read off-camera with you when it was your close-up. Some actors don t do that, but coming from theatre, she always made a point of being there for her fellow actors. Star Trek You ve appeared inRead MoreCharacter Analysis Of Lou Bloom - Ambitious Man831 Words   |  4 Pagessurrounded by food and wine like the gods of Olympus. LOU ...by the only kind of guys that can actually get you that kind of money: sixty-two million, seven-hundred thousand dollars. I don t know all the details. NICKY Matter of fact... ...nobody knew all the details, but it should a been perfect. I mean, he had me, Nicky Santoro, his best friendRead MoreWhere Do I Start?895 Words   |  4 Pageshim the center of attention, constantly talk about him like I m some sort of sycophantic wing-man (basically sit there waste my time and talk all about him, like he just saved the fucking world from impending doom) so he can get pussy, but I didn t so he started to get visibly annoyed by that, mostly because Alanna O Laney s attention towards him somewhat waned as she was on her social media account using his uncle s desktop personal computer. So after a while he started to ramble on and blame

Barclays Matt Barretts Journey

Barclays: Matt Barretts Journey- Winning Hearts and Minds Essay The fact that Barclay is one of the four most dominant retail and commercial banks in the U. K. Says a lot about its reputation. This company has faced many challenges between the time periods of 1980-2000, Matt Barrett has several great ideas to turn this company around, and the doctors that should be considered are essential to the success of his strategy. Social/Demographic The Company initiates good customer relationship skills. They have built personal relationships with one out of five personal customers, and have direct relationships with of the businesses in the LLC. K_ On the downside, the Meanys cost base is much higher than competitors. The plan is to change the core infrastructure Of the company in order to build a stronger customer base. Becoming more competitive and offering value to its core products will give Barclay the competitive edge it needs to help increase profit and attract new clients. Technological Barrett and his Executive Committee (Exec) team have plans to restructure the company by shifting its culture. In order to shift the culture, the mind-set of the employees has to change. Barrett promoted younger talent to join his EXEC team to give the company fresh innovative ideas. These new ideas will give solutions to replace the incompetence of traditional strategies that are no longer useful. The company also did an analysis on the industry and company data to figure out which segments were making money, and which ones werent, A value- based management approach gives the EXEC team an opportunity to take on challenges and risks, and make strategic decision about what segments to add, and what to take away. Economic Considering that Barclay is a reputable company, and the fact that an estimated 20% of its profits are generated outside of the LLC. K; it would be ideal for the many to expand to gain exposure. The company is the ninth largest bank in Europe, and already has a customer base in the country. Developing a second home market in continental Europe would allow the company to create a larger customer base, therefore increasing profit. Environmental/Geographic Barclay is located in a stable economy with a strong domestic franchise, The only crutch is that they need a strategy to compete with new entrants. The LLC. K. Accounts for of the companys profit, so plans to restructure core products will require a dynamic strategic plan. Using diverse tactics to differentiate reduce/customer markets will help aid in maximizing the economic value Of the company; and improving these products by offering value to clients will aid in creating a stronger customer base. Political/Legal/Governmental Barclay is the first foreign bank to file with the Securities and Exchange Commission in Washington DC. This is an advantage to the company on a global scale because banking laws are enforced according to SEC regulations. On the downside, the company may be overlooked by firms who are looking to establish offshore accounts in order to bypass these regulations,

Barclays Matt Barretts Journey

Barclays: Matt Barretts Journey- Winning Hearts and Minds Essay The fact that Barclay is one of the four most dominant retail and commercial banks in the U. K. Says a lot about its reputation. This company has faced many challenges between the time periods of 1980-2000, Matt Barrett has several great ideas to turn this company around, and the doctors that should be considered are essential to the success of his strategy. Social/Demographic The Company initiates good customer relationship skills. They have built personal relationships with one out of five personal customers, and have direct relationships with of the businesses in the LLC. K_ On the downside, the Meanys cost base is much higher than competitors. The plan is to change the core infrastructure Of the company in order to build a stronger customer base. Becoming more competitive and offering value to its core products will give Barclay the competitive edge it needs to help increase profit and attract new clients. Technological Barrett and his Executive Committee (Exec) team have plans to restructure the company by shifting its culture. In order to shift the culture, the mind-set of the employees has to change. Barrett promoted younger talent to join his EXEC team to give the company fresh innovative ideas. These new ideas will give solutions to replace the incompetence of traditional strategies that are no longer useful. The company also did an analysis on the industry and company data to figure out which segments were making money, and which ones werent, A value- based management approach gives the EXEC team an opportunity to take on challenges and risks, and make strategic decision about what segments to add, and what to take away. Economic Considering that Barclay is a reputable company, and the fact that an estimated 20% of its profits are generated outside of the LLC. K; it would be ideal for the many to expand to gain exposure. The company is the ninth largest bank in Europe, and already has a customer base in the country. Developing a second home market in continental Europe would allow the company to create a larger customer base, therefore increasing profit. Environmental/Geographic Barclay is located in a stable economy with a strong domestic franchise, The only crutch is that they need a strategy to compete with new entrants. The LLC. K. Accounts for of the companys profit, so plans to restructure core products will require a dynamic strategic plan. Using diverse tactics to differentiate reduce/customer markets will help aid in maximizing the economic value Of the company; and improving these products by offering value to clients will aid in creating a stronger customer base. Political/Legal/Governmental Barclay is the first foreign bank to file with the Securities and Exchange Commission in Washington DC. This is an advantage to the company on a global scale because banking laws are enforced according to SEC regulations. On the downside, the company may be overlooked by firms who are looking to establish offshore accounts in order to bypass these regulations,

Barclays Matt Barretts Journey

Barclays: Matt Barretts Journey- Winning Hearts and Minds Essay The fact that Barclay is one of the four most dominant retail and commercial banks in the U. K. Says a lot about its reputation. This company has faced many challenges between the time periods of 1980-2000, Matt Barrett has several great ideas to turn this company around, and the doctors that should be considered are essential to the success of his strategy. Social/Demographic The Company initiates good customer relationship skills. They have built personal relationships with one out of five personal customers, and have direct relationships with of the businesses in the LLC. K_ On the downside, the Meanys cost base is much higher than competitors. The plan is to change the core infrastructure Of the company in order to build a stronger customer base. Becoming more competitive and offering value to its core products will give Barclay the competitive edge it needs to help increase profit and attract new clients. Technological Barrett and his Executive Committee (Exec) team have plans to restructure the company by shifting its culture. In order to shift the culture, the mind-set of the employees has to change. Barrett promoted younger talent to join his EXEC team to give the company fresh innovative ideas. These new ideas will give solutions to replace the incompetence of traditional strategies that are no longer useful. The company also did an analysis on the industry and company data to figure out which segments were making money, and which ones werent, A value- based management approach gives the EXEC team an opportunity to take on challenges and risks, and make strategic decision about what segments to add, and what to take away. Economic Considering that Barclay is a reputable company, and the fact that an estimated 20% of its profits are generated outside of the LLC. K; it would be ideal for the many to expand to gain exposure. The company is the ninth largest bank in Europe, and already has a customer base in the country. Developing a second home market in continental Europe would allow the company to create a larger customer base, therefore increasing profit. Environmental/Geographic Barclay is located in a stable economy with a strong domestic franchise, The only crutch is that they need a strategy to compete with new entrants. The LLC. K. Accounts for of the companys profit, so plans to restructure core products will require a dynamic strategic plan. Using diverse tactics to differentiate reduce/customer markets will help aid in maximizing the economic value Of the company; and improving these products by offering value to clients will aid in creating a stronger customer base. Political/Legal/Governmental Barclay is the first foreign bank to file with the Securities and Exchange Commission in Washington DC. This is an advantage to the company on a global scale because banking laws are enforced according to SEC regulations. On the downside, the company may be overlooked by firms who are looking to establish offshore accounts in order to bypass these regulations,